NC RNA extraction reagent is the most effective reagent for isolation of total RNA from samples of human, animal, plant, bacterial and viral origin. This unique reagent provides higher yield and quality of isolated RNA than traditional reagents based on the single-step method. NC RNA extraction reagent isolates pure and undegraded RNA that is ready for the following application without DNase treatment.
NC RNA extraction reagent separates RNA from other molecules in a single-step based on the interaction of phenol and guanidine with cellular components. No chloroform-induced phase separation is necessary to obtain pure RNA.
A biological sample is homogenized or lysed in NC RNA extraction reagent. DNA, proteins, polysaccharides and other molecules precipitate out of the homogenate/ lysate by addition of water and removing it by centrifugation. The pure RNA is isolated from the resulting supernatant by alcohol precipitation, followed by washing and solubilization.
- The isolation procedure can be completed in less than one hour. The isolated RNA is ready for use in RT-PCR, qRT-PCR, microarrays, poly A+ selection, northern blotting, RNase protection assay and other molecular biology applications.
- No chloroform-induced phase separation is necessary to obtain pure RNA.
- The NC RNA extraction reagent procedure is performed at room temperature, including centrifugation.
STABILITY/STORAGE: NC RNA extraction reagent is stable, when storing at 4- 30°C, for at least one year.