EBL AM-50 Glutathione consists of glutathione attached through the sulfur to epoxy activated polyacrylate resulting in a 12-atoms spacer and is suitable for affinity purification of GST-fusion proteins and other S-tranferases or glutathione-dependent proteins. EBL AM-50 Glutathione affinity chromatography permits rapid, mild, non-denaturing, and highly selective purification of glutathione-dependent proteins.
EBL AM-50 series were polyacrylate-based medium with high binding capacity, high performance and high flow rate, providing extraordinary flow characteristic, excellent for scaling-up.
Specifications:
| Particle Size (μm) | 50 |
| Pore Size (Å) | 800 |
| Ligand Density | 150-350 μmol GSH/ ml medium |
| Target Protein Binding Capacity (g/ml) | ≈10 mg recombinant glutathione |
| Bead Matrix | Polyacrylate, PS/DVB |
| Recommended Flow Rate (cm/h) | 150-700 |
| pH Stability | 3-13 |
| Pressure (MPa) | 10 |
| Storage buffer | 20% ethanol |
| Storage temperature | +4–30 °C |
Ordering information: