AM-50 Heparin resin

EBL AM-50 Heparin resin is a well established affinity medium designed for capture or intermediate purification steps. This medium is extensively used for industrial purification of several marketed plasma proteins. Industrial purification of antithrombin III, coagulation factors and other plasma proteins. Coupling chemistry gives chemically-stable ligand attachment. Stability of medium solely dictated by heparin ligand. AM-50 Heparin resin is made with monodispersed polymer beads by coupling the ligand molecules to the matrix using chemically stable and oriented coupling giving high binding capacity. The chemical stability of the media is solely dictated by the heparin ligand. This media supported utilization from lab scale to industrial applications.

The proteins including enzymes purified with immobilized heparin have a long list, and some of them are listed below: mast cell proteases, lipoprotein lipase, coagulation enzymes, elastases, extracellular superoxide dismutase, serine protease inhibitors, such as AT3, heparin cofactor 2 and protease nexin, which inactivates serine proteases in the extracellular matrix. Other heparin binding proteins in the extracellular matrix are vitronectin, fibronectin, thrombospondin, laminin, collagen and Growth factors such as fibroblast growth factor (FGF), Schwann cell growth factor, retinal survival factor and homopoietic growth factor.
Particle Size (μm) 50
Pore Size (Å) 800
Ligand Density about 5 mg/mL heparin of porcine origin
Target Protein Binding Capacity (g/ml) 2 mg antithrombin III
Bead Matrix Polyacrylate
Recommended Flow Rate (cm/h) 200-400
pH Stability 4-13
Pressure (MPa) 10
Chemical stability 0.1 M NaOH (1 week at 20°C)
0.05 M sodium acetate
4 M NaCl, pH 4.0
8 M urea
6 M guanidine hydrochloride
Storage buffer 0.05 M sodium acetate containing 20% ethanol
Storage temperature +4–8 °C
Ordering information:

Catalog Number: amm-5005020, 20 ml

Catalog Number: amm-5005100, 100 ml