Hydrophobic Interaction Chromatography

Hydrophobic interaction chromatography (HIC) is a versatile method for the purification and separation of biomolecules based on their surface hydrophobicity. HIC can be used a first purification step or as the final polishing step to remove remaining impurities. EBL HIC resin Pheny, Butyl, and Octyl media substances are separated on the basis of their varying strength of their hydrophobic interaction with hydrophobic groups attached to the uncharged matrix. EBL HIC media has many properties more suitable as a matrix in preparation of chromatographic matrix than agarose‐based beads. EBL HIC medium has the following three features:
  •    High performance
  •    High dynamic binding capacity
  •    High flow rate
All these features are important for chromatography. All the products manufactured in EBL are based on polyacrylate polymer matrix and with high quality and high batch‐to‐batch reproducibility and are ideal for all stages of an operation – from process development through scale‐up and into production.
Particle Size (μm) 50 ± 5 %
Pore Size (Å) 800
Bead Matrix Highly cross‐linked polyacrylate polymer
Ligand Density ≈50 µmol /mL resin
Suggested Flow Rate (cm/h) 150-700
pH Stability 3-13
Pressure (MPa) 10
Chemical Stability 1.0 M NaOH, 30% isopropanol, 70% ethanol, 6 M guanidine-hydrochloride, 30% Acetonitrile, 1mM HCl
Storage buffer 20 % ethanol
Storage temperature +4- 28 °C