Hydrophobic interaction chromatography (HIC) is a versatile method for the purification and separation of biomolecules based on their surface hydrophobicity. HIC can be used a first purification step or as the final polishing step to remove remaining impurities. EBL HIC resin Pheny, Butyl, and Octyl media substances are separated on the basis of their varying strength of their hydrophobic interaction with hydrophobic groups attached to the uncharged matrix. EBL HIC media has many properties more suitable as a matrix in preparation of chromatographic matrix than agarose‐based beads. EBL HIC medium has the following three features:
- High performance
- High dynamic binding capacity
- High flow rate
Specifications:
Particle Size (μm) | 50 ± 5 % |
Pore Size (Å) | 800 |
Bead Matrix | Highly cross‐linked polyacrylate polymer |
Ligand Density | ≈50 µmol /mL resin |
Suggested Flow Rate (cm/h) | 150-700 |
pH Stability | 3-13 |
Pressure (MPa) | 10 |
Chemical Stability | 1.0 M NaOH, 30% isopropanol, 70% ethanol, 6 M guanidine-hydrochloride, 30% Acetonitrile, 1mM HCl |
Storage buffer | 20 % ethanol |
Storage temperature | +4- 28 °C |