Antibody Affinity Chromatography

EBL AM-50 Protein A, G, and A/G are mediums developed with genetically modified proteins with high alkali-tolerance, which capture monoclonal antibodies for large-scale volumes with chromatography. The resins feature a variety that provides the most versatile combination of chromatographic features for rich yields and high-purity purification of whole IgG for mammalian serum samples.
Key Features:
  •   The ligands with high alkali-tolerant (up to 0.1-0.5 NaOH) allow for cleaning-in-place (CIP).
  •   Improved product quality and economy with Up to 100 cycles of lifetime.
  •   High DBC reduces processing period and medium usage, with all the solutions and consumables saved undoubtedly.
  •   High-flow and high performance allows for rapid processing procedure.
  •   Suits for the range form small scale to industry-scale production.
  •   The ligands are recombinantly produced in Escherichia coli without animal material used in the whole process.
  •   The mono-dispersed polymer matrix with 50μm size features this product high flow rate, high performance and high DBC.
Particle Size (μm)  50 ± 5 %
Bead Matrix  Highly cross‐linked polyacrylate   polymer
Ligand Coupling Method  Epoxy
Ligand  highly Alkali-tolerant IG binding   protein in E. coli.
Dynamic Binding Capacity (g/ml)  ≈30 mg human IgG/ml medium
Max. Linear Flow Rate (cm/h)  700 cm/h
pH Stability  3-12
Pressure (MPa)  10
Cleaning-in-place (CIP) stability  0.1-0.5 M NaOH
Storage buffer  20 % ethanol
Storage temperature  +4-8 °C